nadph cofactor Search Results


nadph cofactor  (Anwendung GmbH)
90
Anwendung GmbH nadph cofactor
Nadph Cofactor, supplied by Anwendung GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nadph+cofactor/10__1002_slash_cite__201250041-50-5-24?v=Anwendung+GmbH
Average 90 stars, based on 1 article reviews
nadph cofactor - by Bioz Stars, 2026-08
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nadph regenerating solution of cofactors corning® gentesttm ugt reaction mix  (Corning Life Sciences)
90
Corning Life Sciences nadph regenerating solution of cofactors corning® gentesttm ugt reaction mix
Nadph Regenerating Solution Of Cofactors Corning® Gentesttm Ugt Reaction Mix, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nadph+cofactor/us12024528-2370-22-27?v=Corning+Life+Sciences
Average 90 stars, based on 1 article reviews
nadph regenerating solution of cofactors corning® gentesttm ugt reaction mix - by Bioz Stars, 2026-08
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nadph cofactor  (BioIVT Inc)
90
BioIVT Inc nadph cofactor
(A) Schematic illustration of the workflow for the Mics assay. (a) Up to three compound plates with 1 μL in each well are obtained from Sample Management. (b) The compounds are diluted in 50% AcN. Human liver microsomes diluted in <t>NADPH</t> containing PBS buffer are dispensed into a matching number of deep well plates. (c) The plates are heated <t>to</t> <t>37°C</t> and incubations are subsequently started by addition of diluted compounds. Sampling, pooling and quenching is achieved at different time points on the Hamilton STARplus platform by adding aliquots to crash plates already holding AcN with IS. A blank plate with diluted liver microsomes is prepared separately. (d) Following incubations all plates are centrifuged to pellet the microsomes. (e) Final sampling of cleared supernatants is combined with additional pooling of plate content from the upper and lower parts of each plate into one half of a single analysis plate, that is, each analysis plate holds the content of two crash plates. These samples are diluted in predispensed H O and subsequently detected using UPLC-MS/MS. (B) Illustration of recent data (2019) from a validation of a new batch of cryopreserved human liver microsomes. The slope based on testing of 46 compounds comes out at 0.980 ( r = 0.951) with a 95% CI of 0.913–1.05. (C) Correlation between two independent experiments submitted to the database. When more than two independent replicates were available, we included the first two instances. The slope based on testing of 1,122 compounds comes out at 0.935 ( r = 0.894) with a 95% CI of 0.916–0.954. (D) Correlation between harmonized assays at AstraZeneca Gothenburg ( y axis) and at Pharmaron ( x axis). The comparison is based on 1,313 compounds with a slope of 0.977 ( r = 0.882) and a 95% CI of 0.957–0.996. (E) Manhattan plot following 6 years of operation from June 2013 to April 2019—diclofenac ( black filled diamond ), verapamil ( green filled triangle ), imipramine ( brown filled circle ), phenacetine ( purple unfilled square ), benzydamine ( blue filled triangle ), and metoprolol ( gray unfilled circle ). Mics, assay for metabolic stability in human liver microsomes. Color images are available online.
Nadph Cofactor, supplied by BioIVT Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nadph+cofactor/pmc07567642-309-32-19?v=BioIVT+Inc
Average 90 stars, based on 1 article reviews
nadph cofactor - by Bioz Stars, 2026-08
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cofactor nadph  (Forschungszentrum gmbh)
90
Forschungszentrum gmbh cofactor nadph
(A) Schematic illustration of the workflow for the Mics assay. (a) Up to three compound plates with 1 μL in each well are obtained from Sample Management. (b) The compounds are diluted in 50% AcN. Human liver microsomes diluted in <t>NADPH</t> containing PBS buffer are dispensed into a matching number of deep well plates. (c) The plates are heated <t>to</t> <t>37°C</t> and incubations are subsequently started by addition of diluted compounds. Sampling, pooling and quenching is achieved at different time points on the Hamilton STARplus platform by adding aliquots to crash plates already holding AcN with IS. A blank plate with diluted liver microsomes is prepared separately. (d) Following incubations all plates are centrifuged to pellet the microsomes. (e) Final sampling of cleared supernatants is combined with additional pooling of plate content from the upper and lower parts of each plate into one half of a single analysis plate, that is, each analysis plate holds the content of two crash plates. These samples are diluted in predispensed H O and subsequently detected using UPLC-MS/MS. (B) Illustration of recent data (2019) from a validation of a new batch of cryopreserved human liver microsomes. The slope based on testing of 46 compounds comes out at 0.980 ( r = 0.951) with a 95% CI of 0.913–1.05. (C) Correlation between two independent experiments submitted to the database. When more than two independent replicates were available, we included the first two instances. The slope based on testing of 1,122 compounds comes out at 0.935 ( r = 0.894) with a 95% CI of 0.916–0.954. (D) Correlation between harmonized assays at AstraZeneca Gothenburg ( y axis) and at Pharmaron ( x axis). The comparison is based on 1,313 compounds with a slope of 0.977 ( r = 0.882) and a 95% CI of 0.957–0.996. (E) Manhattan plot following 6 years of operation from June 2013 to April 2019—diclofenac ( black filled diamond ), verapamil ( green filled triangle ), imipramine ( brown filled circle ), phenacetine ( purple unfilled square ), benzydamine ( blue filled triangle ), and metoprolol ( gray unfilled circle ). Mics, assay for metabolic stability in human liver microsomes. Color images are available online.
Cofactor Nadph, supplied by Forschungszentrum gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nadph+cofactor/pmc05440672__ncomms15420___s1-118-0-15?v=Forschungszentrum+gmbh
Average 90 stars, based on 1 article reviews
cofactor nadph - by Bioz Stars, 2026-08
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cofactor (nadph)  (Cayman Chemical)
90
Cayman Chemical cofactor (nadph)
(A) Schematic illustration of the workflow for the Mics assay. (a) Up to three compound plates with 1 μL in each well are obtained from Sample Management. (b) The compounds are diluted in 50% AcN. Human liver microsomes diluted in <t>NADPH</t> containing PBS buffer are dispensed into a matching number of deep well plates. (c) The plates are heated <t>to</t> <t>37°C</t> and incubations are subsequently started by addition of diluted compounds. Sampling, pooling and quenching is achieved at different time points on the Hamilton STARplus platform by adding aliquots to crash plates already holding AcN with IS. A blank plate with diluted liver microsomes is prepared separately. (d) Following incubations all plates are centrifuged to pellet the microsomes. (e) Final sampling of cleared supernatants is combined with additional pooling of plate content from the upper and lower parts of each plate into one half of a single analysis plate, that is, each analysis plate holds the content of two crash plates. These samples are diluted in predispensed H O and subsequently detected using UPLC-MS/MS. (B) Illustration of recent data (2019) from a validation of a new batch of cryopreserved human liver microsomes. The slope based on testing of 46 compounds comes out at 0.980 ( r = 0.951) with a 95% CI of 0.913–1.05. (C) Correlation between two independent experiments submitted to the database. When more than two independent replicates were available, we included the first two instances. The slope based on testing of 1,122 compounds comes out at 0.935 ( r = 0.894) with a 95% CI of 0.916–0.954. (D) Correlation between harmonized assays at AstraZeneca Gothenburg ( y axis) and at Pharmaron ( x axis). The comparison is based on 1,313 compounds with a slope of 0.977 ( r = 0.882) and a 95% CI of 0.957–0.996. (E) Manhattan plot following 6 years of operation from June 2013 to April 2019—diclofenac ( black filled diamond ), verapamil ( green filled triangle ), imipramine ( brown filled circle ), phenacetine ( purple unfilled square ), benzydamine ( blue filled triangle ), and metoprolol ( gray unfilled circle ). Mics, assay for metabolic stability in human liver microsomes. Color images are available online.
Cofactor (Nadph), supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nadph+cofactor/us09534213-458-29-33?v=Cayman+Chemical
Average 90 stars, based on 1 article reviews
cofactor (nadph) - by Bioz Stars, 2026-08
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nadph cofactor  (Microm International GmbH)
90
Microm International GmbH nadph cofactor
(A) Schematic illustration of the workflow for the Mics assay. (a) Up to three compound plates with 1 μL in each well are obtained from Sample Management. (b) The compounds are diluted in 50% AcN. Human liver microsomes diluted in <t>NADPH</t> containing PBS buffer are dispensed into a matching number of deep well plates. (c) The plates are heated <t>to</t> <t>37°C</t> and incubations are subsequently started by addition of diluted compounds. Sampling, pooling and quenching is achieved at different time points on the Hamilton STARplus platform by adding aliquots to crash plates already holding AcN with IS. A blank plate with diluted liver microsomes is prepared separately. (d) Following incubations all plates are centrifuged to pellet the microsomes. (e) Final sampling of cleared supernatants is combined with additional pooling of plate content from the upper and lower parts of each plate into one half of a single analysis plate, that is, each analysis plate holds the content of two crash plates. These samples are diluted in predispensed H O and subsequently detected using UPLC-MS/MS. (B) Illustration of recent data (2019) from a validation of a new batch of cryopreserved human liver microsomes. The slope based on testing of 46 compounds comes out at 0.980 ( r = 0.951) with a 95% CI of 0.913–1.05. (C) Correlation between two independent experiments submitted to the database. When more than two independent replicates were available, we included the first two instances. The slope based on testing of 1,122 compounds comes out at 0.935 ( r = 0.894) with a 95% CI of 0.916–0.954. (D) Correlation between harmonized assays at AstraZeneca Gothenburg ( y axis) and at Pharmaron ( x axis). The comparison is based on 1,313 compounds with a slope of 0.977 ( r = 0.882) and a 95% CI of 0.957–0.996. (E) Manhattan plot following 6 years of operation from June 2013 to April 2019—diclofenac ( black filled diamond ), verapamil ( green filled triangle ), imipramine ( brown filled circle ), phenacetine ( purple unfilled square ), benzydamine ( blue filled triangle ), and metoprolol ( gray unfilled circle ). Mics, assay for metabolic stability in human liver microsomes. Color images are available online.
Nadph Cofactor, supplied by Microm International GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nadph+cofactor/pmc01368145-3-13-42?v=Microm+International+GmbH
Average 90 stars, based on 1 article reviews
nadph cofactor - by Bioz Stars, 2026-08
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nadph cofactor  (Verlag GmbH)
90
Verlag GmbH nadph cofactor
(A) Schematic illustration of the workflow for the Mics assay. (a) Up to three compound plates with 1 μL in each well are obtained from Sample Management. (b) The compounds are diluted in 50% AcN. Human liver microsomes diluted in <t>NADPH</t> containing PBS buffer are dispensed into a matching number of deep well plates. (c) The plates are heated <t>to</t> <t>37°C</t> and incubations are subsequently started by addition of diluted compounds. Sampling, pooling and quenching is achieved at different time points on the Hamilton STARplus platform by adding aliquots to crash plates already holding AcN with IS. A blank plate with diluted liver microsomes is prepared separately. (d) Following incubations all plates are centrifuged to pellet the microsomes. (e) Final sampling of cleared supernatants is combined with additional pooling of plate content from the upper and lower parts of each plate into one half of a single analysis plate, that is, each analysis plate holds the content of two crash plates. These samples are diluted in predispensed H O and subsequently detected using UPLC-MS/MS. (B) Illustration of recent data (2019) from a validation of a new batch of cryopreserved human liver microsomes. The slope based on testing of 46 compounds comes out at 0.980 ( r = 0.951) with a 95% CI of 0.913–1.05. (C) Correlation between two independent experiments submitted to the database. When more than two independent replicates were available, we included the first two instances. The slope based on testing of 1,122 compounds comes out at 0.935 ( r = 0.894) with a 95% CI of 0.916–0.954. (D) Correlation between harmonized assays at AstraZeneca Gothenburg ( y axis) and at Pharmaron ( x axis). The comparison is based on 1,313 compounds with a slope of 0.977 ( r = 0.882) and a 95% CI of 0.957–0.996. (E) Manhattan plot following 6 years of operation from June 2013 to April 2019—diclofenac ( black filled diamond ), verapamil ( green filled triangle ), imipramine ( brown filled circle ), phenacetine ( purple unfilled square ), benzydamine ( blue filled triangle ), and metoprolol ( gray unfilled circle ). Mics, assay for metabolic stability in human liver microsomes. Color images are available online.
Nadph Cofactor, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nadph+cofactor/10__1002_slash_cbic__201402578-274-9-20?v=Verlag+GmbH
Average 90 stars, based on 1 article reviews
nadph cofactor - by Bioz Stars, 2026-08
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cofactor β-nadph  (Biomol GmbH)
90
Biomol GmbH cofactor β-nadph
(A) Schematic illustration of the workflow for the Mics assay. (a) Up to three compound plates with 1 μL in each well are obtained from Sample Management. (b) The compounds are diluted in 50% AcN. Human liver microsomes diluted in <t>NADPH</t> containing PBS buffer are dispensed into a matching number of deep well plates. (c) The plates are heated <t>to</t> <t>37°C</t> and incubations are subsequently started by addition of diluted compounds. Sampling, pooling and quenching is achieved at different time points on the Hamilton STARplus platform by adding aliquots to crash plates already holding AcN with IS. A blank plate with diluted liver microsomes is prepared separately. (d) Following incubations all plates are centrifuged to pellet the microsomes. (e) Final sampling of cleared supernatants is combined with additional pooling of plate content from the upper and lower parts of each plate into one half of a single analysis plate, that is, each analysis plate holds the content of two crash plates. These samples are diluted in predispensed H O and subsequently detected using UPLC-MS/MS. (B) Illustration of recent data (2019) from a validation of a new batch of cryopreserved human liver microsomes. The slope based on testing of 46 compounds comes out at 0.980 ( r = 0.951) with a 95% CI of 0.913–1.05. (C) Correlation between two independent experiments submitted to the database. When more than two independent replicates were available, we included the first two instances. The slope based on testing of 1,122 compounds comes out at 0.935 ( r = 0.894) with a 95% CI of 0.916–0.954. (D) Correlation between harmonized assays at AstraZeneca Gothenburg ( y axis) and at Pharmaron ( x axis). The comparison is based on 1,313 compounds with a slope of 0.977 ( r = 0.882) and a 95% CI of 0.957–0.996. (E) Manhattan plot following 6 years of operation from June 2013 to April 2019—diclofenac ( black filled diamond ), verapamil ( green filled triangle ), imipramine ( brown filled circle ), phenacetine ( purple unfilled square ), benzydamine ( blue filled triangle ), and metoprolol ( gray unfilled circle ). Mics, assay for metabolic stability in human liver microsomes. Color images are available online.
Cofactor β Nadph, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nadph+cofactor/pm26709721-60-1-6?v=Biomol+GmbH
Average 90 stars, based on 1 article reviews
cofactor β-nadph - by Bioz Stars, 2026-08
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nadph cofactor  (Merck KGaA)
90
Merck KGaA nadph cofactor
(A) Schematic illustration of the workflow for the Mics assay. (a) Up to three compound plates with 1 μL in each well are obtained from Sample Management. (b) The compounds are diluted in 50% AcN. Human liver microsomes diluted in <t>NADPH</t> containing PBS buffer are dispensed into a matching number of deep well plates. (c) The plates are heated <t>to</t> <t>37°C</t> and incubations are subsequently started by addition of diluted compounds. Sampling, pooling and quenching is achieved at different time points on the Hamilton STARplus platform by adding aliquots to crash plates already holding AcN with IS. A blank plate with diluted liver microsomes is prepared separately. (d) Following incubations all plates are centrifuged to pellet the microsomes. (e) Final sampling of cleared supernatants is combined with additional pooling of plate content from the upper and lower parts of each plate into one half of a single analysis plate, that is, each analysis plate holds the content of two crash plates. These samples are diluted in predispensed H O and subsequently detected using UPLC-MS/MS. (B) Illustration of recent data (2019) from a validation of a new batch of cryopreserved human liver microsomes. The slope based on testing of 46 compounds comes out at 0.980 ( r = 0.951) with a 95% CI of 0.913–1.05. (C) Correlation between two independent experiments submitted to the database. When more than two independent replicates were available, we included the first two instances. The slope based on testing of 1,122 compounds comes out at 0.935 ( r = 0.894) with a 95% CI of 0.916–0.954. (D) Correlation between harmonized assays at AstraZeneca Gothenburg ( y axis) and at Pharmaron ( x axis). The comparison is based on 1,313 compounds with a slope of 0.977 ( r = 0.882) and a 95% CI of 0.957–0.996. (E) Manhattan plot following 6 years of operation from June 2013 to April 2019—diclofenac ( black filled diamond ), verapamil ( green filled triangle ), imipramine ( brown filled circle ), phenacetine ( purple unfilled square ), benzydamine ( blue filled triangle ), and metoprolol ( gray unfilled circle ). Mics, assay for metabolic stability in human liver microsomes. Color images are available online.
Nadph Cofactor, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nadph+cofactor/pm36639058-130-14-15?v=Merck+KGaA
Average 90 stars, based on 1 article reviews
nadph cofactor - by Bioz Stars, 2026-08
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cofactor solution nadph  (Carl Roth GmbH)
90
Carl Roth GmbH cofactor solution nadph
(A) Schematic illustration of the workflow for the Mics assay. (a) Up to three compound plates with 1 μL in each well are obtained from Sample Management. (b) The compounds are diluted in 50% AcN. Human liver microsomes diluted in <t>NADPH</t> containing PBS buffer are dispensed into a matching number of deep well plates. (c) The plates are heated <t>to</t> <t>37°C</t> and incubations are subsequently started by addition of diluted compounds. Sampling, pooling and quenching is achieved at different time points on the Hamilton STARplus platform by adding aliquots to crash plates already holding AcN with IS. A blank plate with diluted liver microsomes is prepared separately. (d) Following incubations all plates are centrifuged to pellet the microsomes. (e) Final sampling of cleared supernatants is combined with additional pooling of plate content from the upper and lower parts of each plate into one half of a single analysis plate, that is, each analysis plate holds the content of two crash plates. These samples are diluted in predispensed H O and subsequently detected using UPLC-MS/MS. (B) Illustration of recent data (2019) from a validation of a new batch of cryopreserved human liver microsomes. The slope based on testing of 46 compounds comes out at 0.980 ( r = 0.951) with a 95% CI of 0.913–1.05. (C) Correlation between two independent experiments submitted to the database. When more than two independent replicates were available, we included the first two instances. The slope based on testing of 1,122 compounds comes out at 0.935 ( r = 0.894) with a 95% CI of 0.916–0.954. (D) Correlation between harmonized assays at AstraZeneca Gothenburg ( y axis) and at Pharmaron ( x axis). The comparison is based on 1,313 compounds with a slope of 0.977 ( r = 0.882) and a 95% CI of 0.957–0.996. (E) Manhattan plot following 6 years of operation from June 2013 to April 2019—diclofenac ( black filled diamond ), verapamil ( green filled triangle ), imipramine ( brown filled circle ), phenacetine ( purple unfilled square ), benzydamine ( blue filled triangle ), and metoprolol ( gray unfilled circle ). Mics, assay for metabolic stability in human liver microsomes. Color images are available online.
Cofactor Solution Nadph, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nadph+cofactor/pm28489379-696-11-18?v=Carl+Roth+GmbH
Average 90 stars, based on 1 article reviews
cofactor solution nadph - by Bioz Stars, 2026-08
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nadph cofactor  (ACADEMIC PRESS INC)
90
ACADEMIC PRESS INC nadph cofactor
(A) Schematic illustration of the workflow for the Mics assay. (a) Up to three compound plates with 1 μL in each well are obtained from Sample Management. (b) The compounds are diluted in 50% AcN. Human liver microsomes diluted in <t>NADPH</t> containing PBS buffer are dispensed into a matching number of deep well plates. (c) The plates are heated <t>to</t> <t>37°C</t> and incubations are subsequently started by addition of diluted compounds. Sampling, pooling and quenching is achieved at different time points on the Hamilton STARplus platform by adding aliquots to crash plates already holding AcN with IS. A blank plate with diluted liver microsomes is prepared separately. (d) Following incubations all plates are centrifuged to pellet the microsomes. (e) Final sampling of cleared supernatants is combined with additional pooling of plate content from the upper and lower parts of each plate into one half of a single analysis plate, that is, each analysis plate holds the content of two crash plates. These samples are diluted in predispensed H O and subsequently detected using UPLC-MS/MS. (B) Illustration of recent data (2019) from a validation of a new batch of cryopreserved human liver microsomes. The slope based on testing of 46 compounds comes out at 0.980 ( r = 0.951) with a 95% CI of 0.913–1.05. (C) Correlation between two independent experiments submitted to the database. When more than two independent replicates were available, we included the first two instances. The slope based on testing of 1,122 compounds comes out at 0.935 ( r = 0.894) with a 95% CI of 0.916–0.954. (D) Correlation between harmonized assays at AstraZeneca Gothenburg ( y axis) and at Pharmaron ( x axis). The comparison is based on 1,313 compounds with a slope of 0.977 ( r = 0.882) and a 95% CI of 0.957–0.996. (E) Manhattan plot following 6 years of operation from June 2013 to April 2019—diclofenac ( black filled diamond ), verapamil ( green filled triangle ), imipramine ( brown filled circle ), phenacetine ( purple unfilled square ), benzydamine ( blue filled triangle ), and metoprolol ( gray unfilled circle ). Mics, assay for metabolic stability in human liver microsomes. Color images are available online.
Nadph Cofactor, supplied by ACADEMIC PRESS INC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nadph+cofactor/pm08920937-5-15-22?v=ACADEMIC+PRESS+INC
Average 90 stars, based on 1 article reviews
nadph cofactor - by Bioz Stars, 2026-08
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Image Search Results


(A) Schematic illustration of the workflow for the Mics assay. (a) Up to three compound plates with 1 μL in each well are obtained from Sample Management. (b) The compounds are diluted in 50% AcN. Human liver microsomes diluted in NADPH containing PBS buffer are dispensed into a matching number of deep well plates. (c) The plates are heated to 37°C and incubations are subsequently started by addition of diluted compounds. Sampling, pooling and quenching is achieved at different time points on the Hamilton STARplus platform by adding aliquots to crash plates already holding AcN with IS. A blank plate with diluted liver microsomes is prepared separately. (d) Following incubations all plates are centrifuged to pellet the microsomes. (e) Final sampling of cleared supernatants is combined with additional pooling of plate content from the upper and lower parts of each plate into one half of a single analysis plate, that is, each analysis plate holds the content of two crash plates. These samples are diluted in predispensed H O and subsequently detected using UPLC-MS/MS. (B) Illustration of recent data (2019) from a validation of a new batch of cryopreserved human liver microsomes. The slope based on testing of 46 compounds comes out at 0.980 ( r = 0.951) with a 95% CI of 0.913–1.05. (C) Correlation between two independent experiments submitted to the database. When more than two independent replicates were available, we included the first two instances. The slope based on testing of 1,122 compounds comes out at 0.935 ( r = 0.894) with a 95% CI of 0.916–0.954. (D) Correlation between harmonized assays at AstraZeneca Gothenburg ( y axis) and at Pharmaron ( x axis). The comparison is based on 1,313 compounds with a slope of 0.977 ( r = 0.882) and a 95% CI of 0.957–0.996. (E) Manhattan plot following 6 years of operation from June 2013 to April 2019—diclofenac ( black filled diamond ), verapamil ( green filled triangle ), imipramine ( brown filled circle ), phenacetine ( purple unfilled square ), benzydamine ( blue filled triangle ), and metoprolol ( gray unfilled circle ). Mics, assay for metabolic stability in human liver microsomes. Color images are available online.

Journal: Assay and Drug Development Technologies

Article Title: A Fully Integrated Assay Panel for Early Drug Metabolism and Pharmacokinetics Profiling

doi: 10.1089/adt.2020.970

Figure Lengend Snippet: (A) Schematic illustration of the workflow for the Mics assay. (a) Up to three compound plates with 1 μL in each well are obtained from Sample Management. (b) The compounds are diluted in 50% AcN. Human liver microsomes diluted in NADPH containing PBS buffer are dispensed into a matching number of deep well plates. (c) The plates are heated to 37°C and incubations are subsequently started by addition of diluted compounds. Sampling, pooling and quenching is achieved at different time points on the Hamilton STARplus platform by adding aliquots to crash plates already holding AcN with IS. A blank plate with diluted liver microsomes is prepared separately. (d) Following incubations all plates are centrifuged to pellet the microsomes. (e) Final sampling of cleared supernatants is combined with additional pooling of plate content from the upper and lower parts of each plate into one half of a single analysis plate, that is, each analysis plate holds the content of two crash plates. These samples are diluted in predispensed H O and subsequently detected using UPLC-MS/MS. (B) Illustration of recent data (2019) from a validation of a new batch of cryopreserved human liver microsomes. The slope based on testing of 46 compounds comes out at 0.980 ( r = 0.951) with a 95% CI of 0.913–1.05. (C) Correlation between two independent experiments submitted to the database. When more than two independent replicates were available, we included the first two instances. The slope based on testing of 1,122 compounds comes out at 0.935 ( r = 0.894) with a 95% CI of 0.916–0.954. (D) Correlation between harmonized assays at AstraZeneca Gothenburg ( y axis) and at Pharmaron ( x axis). The comparison is based on 1,313 compounds with a slope of 0.977 ( r = 0.882) and a 95% CI of 0.957–0.996. (E) Manhattan plot following 6 years of operation from June 2013 to April 2019—diclofenac ( black filled diamond ), verapamil ( green filled triangle ), imipramine ( brown filled circle ), phenacetine ( purple unfilled square ), benzydamine ( blue filled triangle ), and metoprolol ( gray unfilled circle ). Mics, assay for metabolic stability in human liver microsomes. Color images are available online.

Article Snippet: The first assay step involves preparation of a homogeneous solution of HLMs, starting with thawing of commercial preparations from BioIVT at 37°C on a water bath and subsequent dilution in buffer with NADPH added as cofactor.

Techniques: Sampling, Tandem Mass Spectroscopy, Biomarker Discovery, Comparison